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The primary cilium has gone from being a vestigial organelle to a crucial signaling hub of growing interest given the association between a group of human disorders, collectively known as ciliopathies, and defects in its structure or function. In recent years many ciliogenesis proteins have been observed at extraciliary sites in cells and likely perform cilium-independent functions ranging from regulation of the cytoskeleton to vesicular trafficking. Perhaps the most striking example is the non-ciliated T lymphocyte, in which components of the ciliary machinery are repurposed for the assembly and function of the immunological synapse even in the absence of a primary cilium. Furthermore, the specialization traits described at the immunological synapse are similar to those seen in the primary cilium. Here, we review common regulators and features shared by the immunological synapse and the primary cilium that document the remarkable homology between these structures.

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Cell-free Macromolecular Synthesis

Serial Number Alcohol 120 1.9 6 - Colaboratory

Cell-free gene expression: an expanded repertoire of applications

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The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. - Abstract - Europe PMC

Effect of calendar ageing on the cycle life of anode-free full-cells.

Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect

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