Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

An illustration of the full-duplex cell-free massive MIMO system.

MitoQuicLy: A high-throughput method for quantifying cell-free DNA from human plasma, serum, and saliva - ScienceDirect

Cancers, Free Full-Text

Cell-free protein synthesis for producing 'difficult-to-express' proteins - ScienceDirect

Mol Imaging Radionucl Ther on X: The Role of a Bone SPECT/CT Scan in the Follow-up of a Solitary Bone Lesion in a Patient with Langerhans' Cell Histiocytosis You can see the

Antibodies, Free Full-Text

The emerging impact of cell-free chemical biosynthesis - ScienceDirect

Cell-free synthesis of human interferon. - Abstract - Europe PMC

Cell-free Fetal DNA — A Trigger for Parturition

Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect

Extracellular histones, cell-free DNA, or nucleosomes: differences in immunostimulation

Cells, Free Full-Text

Cells, Free Full-Text

Towards reproducible cell-free systems

Labile coat: reason for noninfectious cell-free varicella-zoster virus in culture. - Abstract - Europe PMC