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Mario's had one primary nemesis since the NES days, and that nemesis is none other than Bowser. Bowser is the King of the Koopas, a race of evil turtles

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Smash Bros. DOJO!!

Super Smash Bros. Brawl - wii - Walkthrough and Guide - Page 3 - GameSpy

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Mushroomy Kingdom - Super Smash Bros. Ultimate Guide - IGN

Events - Smash Bros Brawl Guide - IGN

Super Smash Bros Brawl - Classic Mode - Bowser

Bowser - Super Smash Bros. for Wii U / 3DS Guide - IGN

Bowser - Super Smash Bros. for Wii U / 3DS Guide - IGN

Super Smash Bros. for Wii U / 3DS Guide - IGN